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Reference: Bresler et al., Mol. Cell. Neurosci. 18:149-167 (2001) 
SAP90/PSD-95:GFP clusters are dendritic and are associated with functional presynaptic boutons. Retrospective staining of neurons expressing SAP90/PSD-95:GFP (green) with antibodies against the specific dendritic market MAP2 (blue) and GluR1 (red) confirms the dendritic localization of SAP90/PSD-95:GFP puncta.. Bars =5μm.
FIG. 2. SAP90/PSD-95:GFP clusters are postsynaptic and are associated with functional presynaptic boutons. (A) Retrospective staining of neurons expressing SAP90/PSD-95:GFP with antibodies against the AMPA-type glutamate receptor subunit GluR1. Note the excellent correspondence between clusters of SAP90/PSD-95:GFP and GluR1, suggesting that most SAP90/PSD-95:GFP clusters are localized to glutamatergic synapses. (B) Retrospective staining of neurons expressing SAP90/PSD-95:GFP (green) with antibodies against the specific dendritic marker MAP2 (blue) confirms the dendritic localization of SAP90/PSD-95:GFP punta. (C) Labeling of functional presynaptic boutons with FM 4-64 (red) reveals that most SAP90/PSD-95:GFP clusters (green) are associated with functional presynaptic boutons. Bars, 10um (A), 5um (B,C)
